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. 2017 Feb 9;8(13):21362–21379. doi: 10.18632/oncotarget.15214

Figure 2. Ectopic expression of miR-34a inhibits the cell migration and invasion in BT-549 BC cells.

Figure 2

A. The BT-549 cells were transfected with plasmid pre-miR-34a and pre-miR-34a-controls as indicated. The miR-34a expression which measured by stem-loop qRT-PCR in transfected cells was significantly increased. B. The relative expression of EMT-TFs in BT-549 cells transfected with miR-34a mimic were analyzed by qRT-PCR. C. Cells were treated the same as above and protein lysis were further analyzed by Western blot assay for the protein expression of EMT-TFs. The over-expressed miR-34a cells reduced NOTCH1, TWIST1, and ZEB1 levels. Nevertheless, the SLUG and ZEB2 levels are no change. D. miR-34a dependently inhibited, migration and invasive characteristics of BT-549 cells. However, no clear effect on cancer cell growth. All data were showed with mean ± SD. Similar results were obtained from three independent experiments. The qRT-PCR results normalized to 18S RNA (18S) or U6 snRNA (U6). In Western blot, the β-actin (ACTIN) as internal controls. (* P<0.05).