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. 2017 Feb 9;8(13):21362–21379. doi: 10.18632/oncotarget.15214

Figure 7. Synergic effects of thymoquinone (TQ) and miR-34a on mRNA (top panel) and protein (bottom panel) level expression of EMT-TFs.

Figure 7

TQ (5 μM) treatment for 6h for RT-PCR or 12h for Western blot pre-miRNAs (20 ng of each) treatment for 36 h inhibited the expression of NOTCH1 A., TWIST1 B., and ZEB1 C. in the BT-549 cell line. Results expressed as mean ± SD, and independently repeated with three times. qRT-PCR results normalized to 18S or U6 and compared with the untreated cells as a control. In western blot β-actin (ACTIN) as an internal control (* P<0.05 and ** P<0.001 versus control group).