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. 2017 Feb 16;8(13):21579–21598. doi: 10.18632/oncotarget.15375

Figure 2. GH-excess promotes and GHRKD attenuates human melanoma cell proliferation.

Figure 2

a1. SK-MEL-28, a2. MALME-3M, a3. MDA-MB-435 and a4. SK-MEL-5 cells were treated with increasing doses of hGH for 48 hr. and cell proliferation was estimated using a resazurin-based metabolic assay. A significant increase in cell proliferation was noted at and above 50 ng/mL hGH treatment. b1. SK-MEL-28, b2. MALME-3M, b3. MDA-MB-435 and b4. SK-MEL-5 cells were transfected with 20 nM scramble or GHR-siRNA for 24 hr. and grown for 48 hr. in presence or absence of 50 ng/mL hGH. Cell proliferation was estimated using resazurin-based metabolic assay. A significant decrease in cell proliferation was noted following GHRKD. Averages of at least four independent experiments performed in quadruplicate were taken. [*, p < 0.05, Students t-test].