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. 2017 Feb 16;8(13):21579–21598. doi: 10.18632/oncotarget.15375

Figure 6. GH-excess increases and GHRKD decreases HGF, MET, ERBB3 RNA-levels in human melanoma cells.

Figure 6

Relative RNA levels of MET (a1, b1, c1, d1), HGF (a2, b2, c2, d2), and ERBB3 (a3, b3, c3, d3), following RT-qPCR of RNA extracted from SK-MEL-28 (a), MALME-3M (b), MDA-MB-435 (c) and SK-MEL-5 (d) cells following addition of 0, 5, 50 and 150 ng/mL hGH or following GHRKD, in presence or absence of 0 and 50 ng/mL hGH. SOCS2 (a4, b4, c4, d4), was used as an internal control for GH action. Results are discussed in the text. In all cases, exogenous hGH treatment was for 24 hr. RNA levels were normalized against expression of β-actin and GAPDH as reference genes. [*, p < 0.05, Wilcoxon sign rank test, n = 4].