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. 2017 Feb 20;8(13):21674–21691. doi: 10.18632/oncotarget.15535

Figure 1.

Figure 1

A. Effects of genistein on the viability of B16F10 cells as determined by WST-8 assay. B16F10 cells were incubated with different concentrations of genistein for 24 and 48 h. B. Cell numbers were counted in the 6-well plates after incubation with genistein for 24 and 48 h. C. Long-term colony formation assay of B16F10 cells after treatment with genistein (0, 12.5, 25, 50, and 100 μM). Cells were grown in the absence or presence of genistein at the indicated concentrations for 7 days. Cells were fixed and stained with crystal violet. Data shown are the averages of three replicates; the experiment was repeated three times. *p<0.05, **p<0.01, compared with the DMSO control. Scale bar is 200 μm.