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. 2017 Feb 24;8(13):21861–21870. doi: 10.18632/oncotarget.15668

Figure 1. Methylation and expression status of HOXA11 in RCC cell lines.

Figure 1

A. The mRNA expression and promoter methylation of HOXA11 was detected by RT-PCR and MSP in RCC cell lines, — represents negative control; BGS analysis of HOXA11 promoter methylation in HEK-293 cell; B. Detection of HOXA11 expression by RT-PCR after demethylation treatment with Aza or Aza +TSA, A: Aza, T: TSA; C. Demethylation treatment induced demethylation in RCC cell lines by MSP, M: mehylation, U: unmethylation; D. BGS analysis of HOXA11 promoter methylation after demethylation treatment, filled circles: methylated CpG site, open circles: unmethylated CpG site.