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. 2017 Feb 24;8(13):21884–21891. doi: 10.18632/oncotarget.15670

Figure 2. Silence of endogenous SPC24 by siRNA suppressed the proliferation of ATC.

Figure 2

(A) and (B) SW1736 and K18 cells were transfected with siRNA against SPC24 or the negative control (non-targeting) siN for 72 hours. Real time PCR (A) or western blot analysis (B) was done in SW1736 and K18 cells. Actin serves the loading control. (C) After 72 hours post tansfection with siRNA against SPC24 or the negative control (non-targeting) siN, SW1736 and K18 cell viability was monitored by MTT assay at the indicated times. (D, E) Soft-agar colony formation for SW1736 and K18 cells transfected with scrambled siRNA (negative control, siN) and SPC24-siRNA, respectively. (Scale bar, 100μm; original magnification,×10). *p < 0.05, **p < 0.01.