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. 2017 Feb 24;8(13):21884–21891. doi: 10.18632/oncotarget.15670

Figure 3. Downregulation of SPC24 induced the cell cycle arrest and promoted cell apoptosis.

Figure 3

(A) and (B) Cell cycle analyses were performed in SW1736 cells (A) and K18 (B) cells transfected with scrambled siRNA (negative control) and SPC24-siRNA by flow cytometry. (C) and (D) SW1736 and K18 cells were collected and analysed for annexin-V and PI labelling. The percentage of cells that were labelled with annexin-V (primary apoptosis) and with annexin-V plus PI (secondary apoptosis) was measured. The fraction of the total percentages that showed only annexin-V labeling and that showed annexin-V plus PI labelling are shown. (E) Quantify analysis of the apoptotic cells was shown in SW1736 and K18 cells transfected with siRNA and SPC24-siRNA. (F) Cells were treated with scrambled siRNA (control) or SPC24 siRNA for 72 hrs and then collected for Western Blot analysis. Cells were lysed and analysed by immunoblotting with the antibodies against PARP, and SPC24. *p < 0.05, **p < 0.01.