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. 2017 Feb 24;8(13):21884–21891. doi: 10.18632/oncotarget.15670

Figure 4. SPC24 increased ATC cell invasive ability and mesenchymal attributes.

Figure 4

(A) and (B) SW1736 and K18 cells were transduced with SPC24 siRNA or scrambled siRNA and 72h post-infection a transwell invasion assay was performed. The cells were allowed to migrate for 24h at 37°C. Images were captured by a digital camera and magnified by ×250. (Scale bar, 100μm; original magnification, ×10). (C) Quantitative assay were performed in SW1736 and K18 cells transduced with SPC24 siRNA or scrambled siRNA. (D) Cells were treated with SPC24 siRNA for 72 hrs and then collected for Western Blot analysis. Cells were lysed and analysed by immunoblotting with the antibodies against E-cadherin, and SPC24. *p < 0.05, **p < 0.01.