Figure 5. SPC24 promoted tumor initiation in xenograft mouse model and in human samples.

(A) Effects of SPC24 downregulation on growth of K18 subcutaneous xenografts in vivo. Nude mice were injected with 2×10⁶ cells and tumors were removed at three weeks post injection; each experimental group contained 4 tumors. (B) The weight of tumors in shSPC24 cells was markedly reduced in comparison with that in shN cells. (C) IHC analysis of SPC24 and E-cadherin expression in the mice tumor tissues. Asterisk (*) refers to non-specific bands. *p < 0.05.