Figure 3. Specific biodistribution of [¹¹¹In]DOTA-Ts29.2 in SW480 and SW480-TSPAN8 bearing mice.

Difference in TSPAN8 expression was assessed by western blot analysis in the two cell lines, SW480 expressing faint basal level of TSPAN8 and transduced SW480-TSPAN8 expressing high levels of TSPAN8 A. Mice bearing both tumors (SW480 on the right side and SW480-TSPAN8 on the left side) were injected (i.v.) with 3.7 MBq of [¹¹¹In]DOTA-Ts29.2 and imaged with a γ-camera at 4 h, 24 h, 72 h, 120 h and 168 h post injection B. Tumors, blood and organs were collected and weighted (3 mice/time point) and the radioactivity was measured by γ-counting of each sample. The graph represents the % of injected activity per gram of tissue (%IA/g) C.