Molecular transmission network of recently acquired HCV infection in Australia (excluding unconnected individuals) constructed with HCV nucleotide sequences from a 1104 bp region encompassing the Core to E2 (minus HVR1) region. An edge was created between any pair of sequences whose divergence was ≤0.03 substitutions/site. Only clustered individuals (nodes) within the network are shown (28%). Oval shaped nodes denote female sex, square shaped nodes denote male sex, and hexagonal shaped nodes denote unknown gender. Nodes are coloured according to participant’s history of injecting drug use (IDU) (blue, never IDU; green, ever IDU). Filled nodes denote HCV/HIV-coinfection; unfilled nodes denote HCV mono infection. The study of origin of participants is denoted by the initials P (HITS-P, prison), C (HITS-C, community) & A (ATAHC) in the node. The direction of edges is determined by estimated date of infection, with an arrow pointing to the most recent estimated date of infection. A threshold was placed on direction of edges where any pair of nodes that had estimated date of infection within 90 days of each other, the edge remained undirected.