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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Immunity. 2017 Mar 14;46(3):405–420. doi: 10.1016/j.immuni.2017.02.018

Figure 1. Immunophenotype of Tax1bp1−/− Mice and Proximal T Cell Functions.

Figure 1

(A) Quantification of total splenocytes (left panel), splenic T cells (TCRb+) and B cells (CD19+) (middle panel), and myeloid cells (Mac-1+), and NK cells (NK1.1+ CD3−) from untreated WT and Tax1bp1−/− mice. Error bars reflect mean values ± SD.

(B) Representative flow cytometry (upper panel) and quantification (lower panel) of splenic CD4+ and CD8+ T cells from WT and Tax1bp1−/− mice.

(C) Flow cytometric analysis of CD62L and CD44 expression on CD4+ (upper panels) and CD8+ T cells (lower panels) from indicated mice.

(D) Immunoblot analyses of signaling molecules from CD4+ T cells from indicated mice at indicated times after anti-CD3 stimulation.

(E) Fluorometric analysis of acute calcium signaling in CD4+ T cells from indicated mice. Data are representative of two experiments.

(F) Intracellular quantification of TNF-α (left graph) and IL-2 (right graph) production 7 h and 19 h, respectively, after anti-CD3 and anti-CD28 stimulation. Mean values ± SD.

(G) Cell surface expression of activation markers, CD69 and CD25, 24 h after anti-CD3 and anti-CD28 stimulation. Unless otherwise noted, all data presented here and hereafter are representative of at least three independent experiments. Please see also Figure S1.