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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: Immunity. 2017 Mar 14;46(3):405–420. doi: 10.1016/j.immuni.2017.02.018

Figure 4. TAX1BP1 Supports mTOR Signaling in T Cells.

Figure 4

(A) Mitochondrial content in resting and activated Tax1bp1+/− and Tax1bp1−/− CD4+ T cells assessed by MitoTracker Green labeling. Cells were stimulated with anti-CD3 and anti-CD28 antibodies. Data are representative of two experiments.

(B) Mitochondrial ROS in resting and activated WT and Tax1bp1−/− T cells assessed by MitoSOX Red labeling in cells stimulated as in (A). Mean values ± SD.

(C) Immunoblot analyses of mTOR signaling proteins in WT and Tax1bp1−/− CD4+ T cells stimulated as in (A).

(D) Real-time quantitative PCR analyses of Eif4bp1 mRNA expression normalized to Hprt mRNA in WT and Tax1bp1−/− CD4+ T cells stimulated as in (A). Mean values ± SD. Data are representative of two experiments.

(E) Immunoblot analyses of AKT phosphorylation (phospho-Thr308 AKT) from stimulated WT and Tax1bp1−/− CD4+ T cells at the indicated time points.

(F) Immunoprecipitation and immunoblot analysis of mTORC1 and mTORC2 complexes in stimulated Tax1bp1+/− and Tax1bp1−/− CD4+ T cells at the indicated time points. Please see also Figure S3.