Elimination of pilus in MGAS10870 liaSR135G increases survival in purified human neutrophils. The survival of MGAS10870 (parental strain), MGAS10870 Δtee3 (parental strain, pilus negative), MGAS10870 liaSR135G (carrier mutant), or MGAS10870 liaSR135G Δtee3 (carrier mutant, pilus negative) was assessed 3 h following interaction with human neutrophils. Percent survival was calculated with the equation (CFU+PMN at 3 h/CFU+PMN at 0 h) × 100. P values were determined by the Mann-Whitney U test.