FIG 1.

IRF6-dependent stimulation of differentiation-associated gene expression in oral keratinocytes by P. gingivalis. (A to D) OKF6 cells were cultured with P. gingivalis (MOI of 100:1) for the times indicated, and IVL (A), KRT13 (B), GRHL3 (C), and OVOL1 (D) mRNA levels were then measured (n = 3). (E to H) OKF6 cells were transfected with an IRF6 (+) or control (–) siRNA. Thereafter, the cells were cultured with P. gingivalis (Pg) (MOI of 100:1) for 24 h, and IVL (E), KRT13 (F), GRHL3 (G), and OVOL1 (H) mRNA levels were then measured (n = 3). **, P < 0.01; *, P < 0.05.