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TLR2-independent stimulation of differentiation-associated gene expression in oral keratinocytes by P. gingivalis. OKF6 cells were transfected with a TLR2 (+) or control (–) siRNA (A to D) or with an IRAK1 (+) or control (–) siRNA (E to H). Thereafter, the cells were cultured with P. gingivalis (MOI of 100:1) for 24 h, and IVL (A and E), KRT13 (B and F), GRHL3 (C and G), and OVOL1 (D and H) mRNA levels were then measured (n = 3). **, P < 0.01; *, P < 0.05.
