FIG 1.

CBU0077 is not required for CCV biogenesis or the intracellular replication of Coxiella. (A, B) Two independent Coxiella Δcbu0077 clones were assessed for their ability to replicate in HeLa cells (A) and THP-1 cells (B) over a 5-day infection period. (A) HeLa cells were infected at an MOI of 100, and lysates of infected cells were collected at days 0, 1, 3, and 5 postinfection to enumerate the Coxiella genome equivalents using qPCR. (B) THP-1 cells were infected at an MOI of 25, and samples were collected at the same time points described above. Graphs represent the fold change in genome equivalents (± standard deviation) relative to those on day 0 for the Coxiella NM PhII wild type (black squares), Δcbu0077 clone 1 (white circles), and Δcbu0077 clone 2 (white triangles) from at least five independent experiments. (C) At day 3 postinfection, HeLa cells were fixed and stained with anti-LAMP-1 (green) and anti-Coxiella (red). *, CCVs. Bars, 10 μm.