FIG 2.

Transfected and translocated CBU0077 proteins demonstrate distinct subcellular localizations. (A) HeLa cells, infected with Coxiella NM PhII and transfected with pFLAG-0077 and pYFP-LAMP-1, demonstrate that ectopically expressed CBU0077 shows lysosomal membrane localization. (B, C) In contrast, ^3×FLAGCBU0077 (B) and the native CBU0077 (C) expressed by Coxiella colocalize with mitochondria stained with MitoTracker Red. (D) This specific mitochondrial localization of CBU0077 was further verified by observing no anti-CBU0077 signal for HeLa cells infected with two independent Coxiella Δcbu0077 strains. These confocal microscopy images are representative of images showing consistently observed localization. YFP, yellow fluorescent protein; *, CCV. Bars, 10 μm.