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. 2017 Apr 21;85(5):e01046-16. doi: 10.1128/IAI.01046-16

FIG 3.

FIG 3

The N-terminal 84 amino acids of MceA can direct mitochondrial localization. (A) The schematic representation demonstrates that MceA is a 263-amino-acid protein with four predicted transmembrane domains (black). Several truncated versions incorporating either the N-terminal or C-terminal transmembrane domain were created and examined for localization. (B to E) HeLa cells persistently infected with Coxiella were transfected with pFLAG constructs to produce 3×FLAG-tagged full-length MceA (MceAFL) (B), MceA85–263 (C), MceA1–144 (D), or MceA1–84 (E). At 24 h posttransfection, cells were incubated for 30 min with MitoTracker Red before being fixed and stained with anti-FLAG and examined with a confocal microscope. The representative images shown here demonstrate that MceAFL and MceA85–263 do not associate with mitochondria; however, the N-terminal fragments of MceA from residues 1 to 144 and 1 to 84 do direct mitochondrial localization. *, CCVs. Bars, 10 μm.