FIG 5.

Extrusion uptake causes activation of caspase 3 and/or 7 in dendritic cells. C57BL/6 BMDCs were exposed to C. trachomatis LGV L2 extrusions or dose-matched free bacteria. At 3 hpe, DCs were stained with the caspase 3/7 probe and imaged. (A and B) Representative images of DCs containing extrusions (A) or DCs exposed to 100 times the free-C. trachomatis EB dose (B) at 3 hpe. Red, caspase 3/7 probe; blue, DAPI; green, C. trachomatis. Scale bar, 5 μm. (C) Quantification of caspase 3/7 probe intensity at 3 hpe. Data are means ± SEM of the results of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.