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. 2005 Jan;43(1):491–493. doi: 10.1128/JCM.43.1.491-493.2005

FIG. 2.

FIG. 2.

SDS-PAGE of exoantigens (2 μg) obtained from 10 subcultures of P. brasiliensis (Pb-18) clones 3, 4, and 6 and of exoantigens (2 μg) obtained from 10 subcultures of Pb-339 clone 1. Observe the variability in gp43 expression among the subcultures. In Pb-18 clone 3, exoantigens numbers 1 and 7 secreted great amounts of gp43. In Pb-18 clone 4, exoantigens 3, 7, and 8 secreted large amounts of gp43. In Pb-18 clone 6, exoantigens 2, 3, and 9 secreted large amounts of gp43. In Pb-339 clone 1, gp43 was in abundance in exoantigens 1 and 2 but began to decrease after 3. S, standard purified gp43. Lanes 1 to 10, exoantigens of consecutive subcultures.