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Immunoblot analysis of purified P1-C1 protein with individual and pooled IgG. Results with positive patient sera and with anti-histidine antibodies are shown. Lanes 2, 19, 27, 17, and 25 represent positive individual sera. Lane 16 represents a negative control (negative individual sera, tested negative by commercial kit). PS, P1-C1 reacted with positive pooled sera; M, standard protein marker; PH, P1-C1 reacted with anti-histidine antibodies.
