TABLE 1.
Detection of CSFV in clinical samples by CSFV real-time RT-PCR assay, VI, and nested PCR
| Farm | Location | CSF clinical signs | No. of samples | Vaccinea | No. of samples
|
CSFV RNA detected by nested PCRb | ||
|---|---|---|---|---|---|---|---|---|
| VI positive | Real-time PCR positive | VI negative PCR positive | ||||||
| 1 | Licey | No | 22 | Marker/subunit | 0 | 2 | 2 | ND (0/2) |
| 2 | Licey | No | 19 | LAV | 0 | 0 | ||
| 3 | Licey | Yes | 57 | None | 3 | 5 | 2 | DR02.3 (2/2) |
| 4 | Licey | No | 18 | LAV | 0 | 0 | ||
| 5 | Licey | Yes | 112 | LAV | 45 | 52 | 7 | DR02.6 (7/7) |
| 6 | Licey | No | 10 | LAV | 0 | 1 | 1 | ND (0/1) |
| 7 | La Vega | Yes | 102 | Marker/subunit | 0 | 1 | 1 | DR02.1 (0/1) |
| 8 | La Paloma | No | 65 | Marker/subunit | 0 | 1 | 1 | ND (1/1) |
| 9 | La Paloma | Yes | 44 | LAV | 2 | 7 | 5 | DR02.6 (1/5); DR02.4 (4/5) |
| Total | 449 | 50 | 69 | 19 | 15/19 | |||
a
“Marker/subunit” indicates that at the time of sample collection a E2 CSFV vaccine was in use, before that live attenuated vaccine had been used. LAV, live attenuated vaccine.
b
Values are number of samples with indicated RNA/number of VI-negative PCR-positive samples. ND, not detected.