TABLE 2.
Performance of the CSFV real-time RT-PCR assay after evaluation of VI-negative CSFV real-time RT-PCR-positive samples by RT-nested PCR
| Test indexa | % | 95% Confidence interval |
|---|---|---|
| Sensitivity | 100 (65/65) | 100-100 |
| Specificity | 98.9 (380/384) | 97.9-99.9 |
| Positive predictive value | 94.3 (65/69) | 88.6-99.7 |
| Negative predictive value | 100 (380/380) | 100-100 |
| False-negative rate | 0.00 (0/65) | 0.0-0.0 |
| False-positive rate | 5.7 (4/69) | 1.8-14.9 |
| Overall accuracy | 99.1 (445/449) | 98.9-99.9 |
a
Sensitivity, number of positive samples over number of true-positive samples; specificity, number of negative samples over number of true-negative samples; positive predictive value, number of samples that were positive and had CSFV; negative predictive value, number of samples that were negative and did not have CSFV; false-negative rate, number of negative samples confirmed positive over true-positive samples; false-positive rate, number of positive samples confirmed negative over true-positive samples; overall accuracy, total of true-positive and true-negative samples over the total number of samples.