Presumptive identification of M. tuberculosis by double digestion (EcoRI and HindII) of cloned constructs. Lanes M, marker DNA (100-bp ladder). (a) Of the 10 plasmid constructs, only the constructs in lanes 4, 5, 7, 8, and 10 harbored the M. tuberculosis rpoB DNA insert. Lanes 1 to 10, plasmids purified from 10 colonies of transformed E. coli. (b) The plasmid constructs in all lanes harbored the M. tuberculosis rpoB DNA insert.