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. 2005 Jan;43(1):174–178. doi: 10.1128/JCM.43.1.174-178.2005

FIG. 2.

FIG. 2.

Presumptive identification of M. tuberculosis by double digestion (EcoRI and HindII) of cloned constructs. Lanes M, marker DNA (100-bp ladder). (a) Of the 10 plasmid constructs, only the constructs in lanes 4, 5, 7, 8, and 10 harbored the M. tuberculosis rpoB DNA insert. Lanes 1 to 10, plasmids purified from 10 colonies of transformed E. coli. (b) The plasmid constructs in all lanes harbored the M. tuberculosis rpoB DNA insert.