TABLE 1.
Nucleotide sequences of PCR primers used in this study
| Genea | Primer sequence (5′ to 3′) | Tm (°C) | Reference |
|---|---|---|---|
| intA(S) | ATC ATC GTC GTA GAG ACG TCG G | 67.4 | 11 |
| intB(AS) | GTC AAG GTT CTG GAC CAG TTG C | 66.9 | 11 |
| blaIMP-1(S) | CTA CCG CAG CAG AGT CTT TG | 62.7 | This study |
| blaIMP-1(AS) | AAC CAG TTT TGC CTT ACC AT | 59.9 | This study |
| blaVIM-2(S) | AAA GTT ATG CCG CAC TCA CC | 63.9 | This study |
| blaVIM-2(AS) | TGC AAC TTC ATG TTA TGC CG | 64.5 | This study |
| aacA4(S) | GAC CTT GCG ATG CTC TAT GAG TGG CTA AAT | 73.0 | This study |
| aacA4(AS) | TTC GCT CGA ATG CCT GGC GTG TT | 76.9 | This study |
| aadA1(S) | TGA TCG CCG AAG TAT CGA CTC | 66.3 | This study |
| aadA1(AS) | CCT TGG TGA TCT CGC CTT TC | 65.8 | This study |
| aadA2(S) | TTC GAA CCA ACT ATC AGA GGT GCT AA | 67.4 | This study |
| aadA2(AS) | AAA GCG AAT AAA TTC TTC CAA GTG ATC T | 66.4 | This study |
| blaOXA-2(S) | CAA TCC GAA TCT TCG CGA TAC TT | 66.9 | This study |
| blaOXA-2(AS) | AAG TAT CGC GAA GAT TCG GAT TG | 66.9 | This study |
| qacEΔ1 | CTC TCT AGA TTT TAA TGC GGA TG | 60.6 | This study |
a
(S), sense; (AS), antisense.