TABLE 1.

Comparison of interassay variation for real-time QT-NASBA and real-time QT-PCR^a

Assay and log input no./ml	Avg interassay variation (range)		Assay and log input no./ml	Avg interassay variation (range)
Real-time QT-NASBA			Real-time QT-PCR
6	4.67 (3.62-6.16)		6	4.51 (1.86-8.75)
5	4.04 (1.09-6.81)		5	3.07 (0.81-6.42)
4	3.39 (2.68-4.29)		4	4.54 (1.17-12.65)
3	4.45 (3.19-6.60)		3	2.08 (1.13-4.13)
2	7.95 (6.01-9.78)		2	3.87 (1.65-8.75b)
1	9.71 (7.48-12.41)		1	8.53 (4.84-10.84c)

^aInterassay variation for ring stage parasite quantification, calculated as standard deviation/mean × 100% for each parasite concentration. The average and range were calculated over three analyses on different days for real-time QT-NASBA and over three duplicate analyses on different days for real-time QT-PCR.

^bn = 4.

^cn = 3.