TABLE 2.
Oligonucleotide primers used for amplification of the urease gene in Helicobacter isolates from cheetahs
| Species specificity | Primer. | Sequence (5′ to 3′) | Size of expected product (bp) |
|---|---|---|---|
| “H. heilmannii” | HhU3351F | CTATCAACTGCGGTTGCGAC | 140 |
| HhU3481R | TCGCCATAAGTGGTGCAGTC | ||
| HhU3351F | CTATCAACTGCGGTTGCGAC | 403 | |
| HhU3716R | TTCTGTAGCAGGTCCTACGC | ||
| H. pylori | HpU1286F | ACGCAACCACTATCACTCC | 115 |
| HpU1400R | CTTGCATCGTTAGAAGCGTTA | ||
| HpU1204F | TCCCCCCAACAAATCCCTAC | 297 | |
| HpU1501R | TGTCCGCAACATCTAACGC |
.
F and R designate forward and reverse primers, respectively.