TABLE 2.
Oligonucleotides used for identification of N. meningitidis
| Laboratory(ies) | Target gene for identification | Oligonucleotide sequences (5′-3′) | Size (bp) |
|---|---|---|---|
| GCTGCGGTAGGTGGTTCAA | |||
| L1, L2, L5 | ctrA | TTGTCGCGGATTTGCAACTA | 111 |
| CATTGCCACGTGTCAGCTGCACAT (probe) | |||
| L3, L7, L9,c L11 | crgA | GCTGGCGCCGCTGGCAACAAAATTC | 230 |
| CTTCTGCAGATTGCGGCGTGCCGT | |||
| IS1106b | ATTATTCAGACCGCCGGCAG | 650 | |
| CCGATAATCAGGCATCCG | |||
| 16S rRNAc | AACT(AC)CGTGCCAGCAGCCGCGGTAA (outer) | ||
| AAGGAGGTGATCCA(AG)CCGCA(GC)(GC)TTC (outer) | 1,031 | ||
| TGTTGGGCAACCTGATTG (seminested) | |||
| AAGGAGGTGATCCA(AG)CCGCA(GC)(GC)TTC (seminested) | 710 | ||
| L4 | 16S rRNA | TGTTGGGCAACCTGATTG | 711 |
| TGATCCA(G/A)CCGCA(G/C)(G/C)TTC | |||
| L6 | 16S rRNA | AGTTTGATCHTGGCTCAGa | 796 |
| GGACTACHAGGGTATCTAAT | |||
| L8c | porA | AAACTTACCGCCCTCGTA (outer) | Variable |
| TTAGAATTTGTGGCGCAAACCGAC (outer) | |||
| CCGCACTGCCGCTTGCGG (nested) | Variable | ||
| CGCATATTTAAAGGCATA (nested) | |||
| L10 | ctrA | TTGTGTGGAAGTTTAATTGTAGGATGC | 89 |
| TCAGATTGTTGCCCTAAAGAGACA | |||
| TCCTTCATCAGGCCCCAGCG (probe) |
a
h = A, C, or T not G.
b
This laboratory additionally used IS1106 for identification.
c
These two laboratories additionally used the same 16S rRNA gene oligonucleotides for identification.