Fig. 1.

Water pipe condensate (WPC) suppress the viability and proliferation of A549 cells. Time-dependent effect of exposure of A549 cells to 4 mg/ml WPC on (a) cell viability as measured by MTT assay and cell proliferation as measured by BrdU assay (b & c). Data were analyzed at 24, 48, 72 and 96 h. The relative cell viability/proliferation (%) was expressed as a percentage relative to that seen in PBS-treated control cells at all time points studied (c). The data are presented as the measured absorbance based on the BrdU incorporation. Data represent mean ± S.E.M. of three independent experiments each repeated in triplicate. *P < 0.05, **P < 0.01 significantly different compared to control and ^^ P < 0.01 significantly different compared to control