Table 1.
List of primer sequences use for first and second round PCR for each og the three HPV genotypes
HPV genotype | First round PCR primers | Second round PCR primers |
---|---|---|
HPV16 | HPV16R1F (5’CACCTACTAATTGTGTTGTGG3’) | HPV16R2F (5’GGGGTACCTCGGTTGCATGCTTTTTGGC3’) |
HPV16R1R (5’GTTTGCACACACCCATG3’) | HPV16R2R (5’GGTCTAGACGGTTTGCACACACCCATGT3’ | |
HPV18 | HPV18R1F (5’GTGTTTGTGGTATGGGTGTT3’) | HPV18R2F (5’CGGTTGCATAAACTATGTAT3’) |
HPV18R1R (5’GTATAGTATGTGCTGCCCAA3’) | HPV18R2R (5’TCGGTTGCCTTTGGCTTATG3’), | |
HPV45 (fragment 1) | HPV45F (5’GACCTCGTAAGCGTCCTGC3’) | HPV45F (5’GACCTCGTAAGCGTCCTGC3’) |
HPV45R (5’GGATGCTGTGTAGTATG CAAGTTTATA3’) | HPV45R (5’GGATGCTGTGTAGTATG CAAGTTTATA3’); | |
HPV45 (fragment 2) | HPV45F2 (5’GTATGGTGTTACTGTACATA ATTGTGG3’) | HPV45F2 (5’GTATGGTGTTACTGTACATA ATTGTGG3’) |
HPV45R2 (5’CATAGGGTGTGGATACAGTTGTG3’) | HPV45R2 (5’CATAGGGTGTGGATACAGTTGTG3’) | |
HPV45 (fragment 3) | HPV45F3 5’CTGGCACATTTACAACCCCTAC3’) | HPV45F3 (5’CTGGCACATTTACAACCCCTAC3’) |
HPV45R3 (5’CGCCATCCTGCAATGCAC 3’) | HPV45R3 (5’CGCCATCCTGCAATGCAC 3’) |