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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Behav Brain Res. 2016 Oct 22;340:63–70. doi: 10.1016/j.bbr.2016.10.037

Figure 3.

Figure 3

Effect of GSNO, HIF-1α inhibitor 2-ME, and denitrosylating agent DDT on the expression of VEGF and BDNF in endothelial (bEnd3) cells. GSNO treatment increased the mRNA expression levels of VEGF and BDNF in a dose dependent manner (A). Pretreatment (2 h) with 2-ME (5 μM) or DDT (200 μM) reversed the effect of GSNO on the expression levels of VEGF and BDNF (C). The expression of VEGF and BDNF was determined at 24 h using RT-PCR and normalized against β-actin. Under the described experimental conditions, cell viability was assayed using LDH release assay (B, D), showing no significant cell death. Data are presented as mean +SD. n=5. ***p<0.001, *p<0.05 vs. Untreated, +++p<0.001 vs. GSNO.