A, Confocal fluorescent microscopy images of diploid strains, containing Pab1-mRuby2 and Pab1-Clover, showing Pab1-mRuby2 after 8-minute incubation at the indicated temperatures. Arrow indicates a stress granule. B, Pab1 western blot after 10-minute heat shock of wild-type cells. Lysed samples were incubated with or without RNase1, then progressively fractionated at 8,000g and 100,000g yielding pellets (P8 and P100, respectively) and remaining supernatant (S). C and D, Quantification of B where each fraction is normalized to the total intensity in all fractions. Red or black indicate RNase1 or buffer treatment, respectively.