TABLE 3.

Normalized fluorescence of each serotype assay in the presence of various dilutions of pneumococcal lysates of cross-reactive serotypes

Assay serotype	Presumed epitope	Cross-reactive serotype	Dilution of lysatesa
			1:5	1:20	1:80	1:320	1:1,280
10A/10B	10d	10A	16.4	18.7	20.3	31.6	37.9
		10B	31.1	42.4	50.7	71.0	86.6
		10C	90.9	98.7	103.2	101.6	105.6
		10F	87.5	95.0	107.0	104.9	108.7
11A/11D/11F	11e	11A	1.0	1.9	6.6	16.1	34.7
		11B	124.3	121.2	119.3	109.6	88.2
		11C	86.5	114.9	106.5	95.6	92.8
		11D	1.4	3.5	7.1	18.2	42.2
		11F	7.9	12.2	22.9	38.8	54.9
12A/12B/12F	12a	12A	19.5	27.9	37.6	56.4	64.8
		12B	12.0	17.1	27.1	43.9	62.8
		12F	1.3	2.4	5.8	12.5	26.8
15B	15h	15A	81.3	88.3	99.2	93.4	97.9
		15B	6.0	8.8	17.8	38.0	56.8
		15C	39.9	60.2	67.0	87.5	85.4
		15F	64.1	79.6	101.1	93.5	109.3
17F	17b	17A	82.5	93.7	85.5	88.9	85.9
		17F	1.7	4.8	11.8	26.4	51.2
22A/22F	22a	22A	26.7	32.9	49.8	59.6	84.0
		22F	4.2	13.6	22.3	44.4	66.4
33A/33F	33b	33A	14.7	21.7	34.5	50.3	68.3
		33B	79.8	91.1	91.8	97.2	100.8
		33C	99.6	106.3	119.6	113.4	106.6
		33D	90.5	92.7	99.1	94.3	93.2
		33F	16.4	23.6	28.6	42.9	60.7

^aThe numbers in the table indicate normalized fluorescence, which was calculated as (geometric mean fluorescence of a sample − geometric mean of background)/(geometric mean fluorescence of negative sample − geometric mean of background). Fluorescence of the beads without any primary antibody was used as the background fluorescence. The fluorescence obtained with the lysate of a noncapsulated strain (R36A) or a strain expressing an unrelated serotype was used as the fluorescence of negative sample.