Hybridization of BamHI-PstI-digested genomic DNA from EHEC O157 strains and controls with the terC probe. M, molecular weight marker (1-kb DNA ladder; Gibco-BRL). In lanes 1 to 7, the following strains are displayed (serotype, Te-MIC in micrograms per milliliter, and the presence of ter genes as detected by PCR are given in parentheses): lane 1, EDL933 (O157:H7, 128, ter+); lane 2, C600 (not available; <1; ter negative) (negative control); lane 3, 5288/91 (O157:H7; <1; ter negative); lane 4, 4180/97 (SF O157:NM; 256; ter negative); lane 5, 493/89 (SF O157:NM; <1; ter negative); lane 6, 3226/98 (SF O157:NM; 128; ter+); lane 7, 3323/98 (SF O157:NM; 128; ter+). Two terC copies detected in EDL933 after DNA separation by pulsed-field gel electrophoresis (19) were not distinguishable by conventional gel electrophoresis.