TABLE 1.
Serotype | Total no. of strains | SFb | No. (%) of strains with a characteristic
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Te MIC (μg/ml) in range of:
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Robust growthc on:
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No or strongly reduced growthd on:
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ter genese
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256-1024 | 32-128 | 2-4 | <1 | CT-SMAC | LB-Te agar | CT-SMAC | LB-Te agar | Present | Absent | |||
O157:H7 | 67 | 0 | 50 (74.6)f | 16 (23.9)f | 0 (0) | 1 (1.5) | 66 (98.5) | 66 (98.5) | 1 (1.5) | 1 (1.5) | 66 (98.5)g | 1 (1.5) |
O157:NMa | 86 | 86 | 1 (1.2)f | 2 (2.3)f | 40 (46.5) | 43 (50.0) | 3 (3.5) | 3 (3.5) | 83 (96.5) | 83 (96.5) | 2h (2.3)g | 84i (97.7) |
NM, nonmotile.
SF, sorbitol fermentation as determined after overnight incubation on SMAC agar.
Robust growth, >1,000 colonies of normal size per plate.
No growth, no colonies grown; strongly reduced growth, <10 colonies reduced in size present after overnight incubation.
As determined by PCRs targeting terZ, terA, terB, terC, terD, terE, and terF (19); present, all genes investigated were present; absent, all genes investigated were absent.
Tellurite resistance (Ter): EHEC O157:H7 versus SF EHEC O157:NM, P < 0.000001 by the χ2 test.
Presence of ter genes: EHEC O157:H7 versus SF EHEC O157:NM, P < 0.000001 by the χ2 test.
Two of three Ter strains; the third was negative for ter genes.
83 Tes strains and 1 Ter strain.