FIG. 1.

cyp51A allele discrimination by molecular beacons. Real-time PCR was used to evaluate the specificity of eight different molecular beacons designed to distinguish the wild-type allele and seven mutant alleles. Each panel shows an individual molecular beacon and its relevant Gly54 allele recognition sequence. Real-time PCRs were performed with separate 500-bp templates corresponding to the GGG (*), GAA (▾), AAG (□), GAG (⋄), AGG (▪), GTG (♦), and TGG (▴) cyp51A alleles and a blank (no DNA; •) with the indicated specific molecular beacons. Each panel shows a composite representation of eight separate template reactions with the same molecular beacon.