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. 2005 Jan;43(1):214–222. doi: 10.1128/JCM.43.1.214-222.2005

FIG. 2.

FIG. 2.

Multiplex assay formats. (A) Graphic output of the Stratagene Mx4000 software for the multiplex single-tube assay. The top semicircle is highlighted when the FAM signal is observed, reporting the presence of the wild-type cyp51A allele. The bottom semicircle is highlighted when the HEX signal is observed, reporting the presence of any of seven mutant cyp51A alleles. (B) Graphic output from the Stratagene Mx4000 software for the multiplex double-tube assay. Each quadrant of the eight total sectors in the two circles represents an allele-specific molecular beacon labeled with HEX, ROX, Q670, or FAM, as indicated. The top right sector of the upper circle is highlighted when the FAM signal is observed, reporting the presence of the wild-type cyp51A allele. All other sectors are highlighted when the HEX, ROX, Q670, or FAM signal is observed, reporting the presence of a specific mutant cyp51A allele.