TABLE 1.
DNA oligonucleotides used in work
| Primer or molecular beacon | Sequencea | 5′ end modification | 3′ end modification | Purpose |
|---|---|---|---|---|
| Af210F | GTCTCTCATTCGTCCTTGTCCT | None | None | Sequencing PCR primer |
| Af709R | CGTTGAGAATAAACTCGTTCCC | None | None | Sequencing PCR primer |
| CYP51AS | TCATTGGGTCCCATTTCTG | None | None | Real-time PCR primer |
| CYP51AA | GCACGCAAAGAAGAACTTG | None | None | Real-time PCR primer |
| AAG-FAM | CGCGATCATCAGTTACAAGATTGATCCATCGCG | FAM | Dabcyl | AAG allele probe |
| GGG-FAM | CGCGATCATCAGTTACGGGATTGATCCATCGCG | FAM | Dabcyl | Wild-type allele probe |
| GAA-HEX | CGCGATCATCAGTTACGAAATTGATCCATCGCG | HEX | Dabcyl | GAA allele probe |
| AAG-HEX | CGCGATCATCAGTTACAAGATTGATCOATCGCG | HEX | Dabcyl | AAG allele probe |
| GAG-HEX | CGCGATCATCAGTTACGAGATTGATCCATCGCG | HEX | Dabcyl | GAG allele probe |
| AGG-HEX | CGCGATCATCAGTTACAGGATTGATCCATCGCG | HEX | Dabcyl | AGG allele probe |
| GTG-HEX | CGCGATCATCAGTTACGTGATTGATCCATCGCG | HEX | Dabcyl | GTG allele probe |
| TGG-HEX | CGCGATCATCAGTTACTGGATTGATCCATCGCG | HEX | Dabcyl | TGG allele probe |
| CGG-HEX | CGCGATCATCAGTTACCGGATTGATCCATCGCG | HEX | Dabcyl | CGG allele probe |
| TGG-ROX | CGCGATCATCAGTTACTGGATTGATCCATCGCG | ROX | Dabcyl | TGG allele probe |
| CGG-ROX | CGCGATCATCAGTTACCGGATTGATCCATCGCG | ROX | Dabcyl | CGG allele probe |
| GAA-Q670 | CGCGATCATCAGTTACGAAATTGATCCATCGCG | Q670 | Dabcyl | GAA allele probe |
| GTG-Q670 | CGCGATCATCAGTTACGTGATTGATCCATCGCG | Q670 | Dabcyl | GTG allele probe |
| GGG-T | AAAAGGATCAATCCCGTAACTGATGAAAA | None | None | Wild-type allele target |
| GAG-T | AAAAGGATCAATCTCGTAACTGATGAAAA | None | None | GAG allele target |
| AAG-T | AAAAGGATCAATCTTGTAACTGATGAAAA | None | None | AAG allele target |
| GAA-T | AAAAGGATCAATTTCGTAACTGATGAAAA | None | None | GAA allele target |
| AGG-T | AAAAGGATCAATCCTGTAACTGATGAAAA | None | None | AGG allele target |
| CGG-T | AAAAGGATCAATCCGGTAACTGATGAAAA | None | None | CGG allele target |
| TGG-T | AAAAGGATCAATCCAGTAACTGATGAAAA | None | None | TGG allele target |
| GTG-T | AAAAGGATCAATCACGTAACTGATGAAAA | None | None | GTG allele target |
a
Probe domains of molecular beacons and target domains of allele targets are underlined. Sequences for molecular beacon probe domains corresponding to codon 54 in A. fumigatus gene cyp51A and complement codons in allele targets are in boldface.