TABLE 2.

T_ms for CYP51A molecular beacons

	Tma at Mg concn of:
Beacon	3 mM for target:								5 mM for target:
	GGG-T	GAA-T	AAG-T	GAG-T	AGG-T	GTG-T	TGG-T	CGG-T	GGG-T	GAA-T	AAG-T	GAG-T	AGG-T	GTG-T	TGG-T	CGG-T
AAG-FAM	48.7	44.7	62.2	52.7	53.2	48.2	50.7	49.7	49.7	45.2	63.7	54.2	54.7	54.2	52.2	52.2
GGG-FAM	65.2	53.7	54.2	58.7	59.7	61.7	58.2	55.7	67.2	55.2	55.7	61.2	61.7	63.7	61.2	59.7
GAA-HEX	45.7	61.2	41.7	50.2	35.2	45.2	41.7	36.2	46.7	63.7	47.7	52.7	41.2	46.7	42.2	41.7
AAG-HEX	46.2	39.7	61.2	52.2	51.7	49.7	48.2	47.2	48.7	44.7	63.2	53.2	53.2	51.2	50.7	49.7
GAG-HEX	55.7	55.7	56.7	63.2	50.2	57.7	50.7	50.7	57.2	56.2	57.2	64.2	51.7	58.7	52.7	52.2
AGG-HEX	54.7	42.2	55.2	49.7	61.7	49.7	56.2	58.2	56.7	44.7	57.2	51.7	63.7	52.2	58.2	59.7
GTG-HEX	54.7	47.7	48.7	54.7	49.7	63.7	51.7	49.7	56.2	48.7	51.2	57.2	50.7	65.2	53.7	52.2
TGG-HEX	56.2	47.2	53.7	52.7	58.7	53.7	63.7	60.2	57.7	48.2	54.7	53.2	59.7	55.2	65.2	61.7
CGG-HEX	54.7	40.7	47.7	48.2	54.2	50.2	54.7	65.2	55.2	42.7	49.2	50.2	55.2	51.7	57.2	66.2
TGG-ROX	52.7	39.2	49.7	46.2	56.2	48.2	62.7	58.2	54.2	39.7	51.2	49.2	57.7	50.2	63.7	59.7
CGG-ROX	52.7	40.7	47.7	46.7	53.7	49.7	53.2	64.2	54.2	42.2	48.7	49.7	54.7	51.2	55.2	65.7
GAA-Q670	42.2	58.2	42.7	48.7	39.7	42.7	39.2	41.2	46.7	61.7	42.7	51.2	40.2	48.2	44.7	43.2
GTG-Q670	44.7	46.2	47.7	55.2	42.7	61.2	49.7	48.2	49.7	48.2	50.2	56.2	49.2	64.2	51.7	50.2

^aT_ms of complement molecular beacon-target hybrids are in boldface.