TABLE 3.
A. fumigatus strains analyzed by multiplex molecular beacon real-time PCR
| Strain | MIC (μg/ml) | 54th codon of cyp51A | Amino acid |
|---|---|---|---|
| H11-20 | 0.5 | GGG | Gly |
| R7-1 | >16a | TGG | Trp |
| RIT7 | >16 | GGG | Gly |
| RIT12 | >16 | GAG | Glu |
| RIT14 | >16 | GGG | Gly |
| RIT15 | >16 | AAG | Lys |
| RIT18 | >16 | GAA | Glu |
| RIT19b | >16 | AGG | Arg |
| RIT27 | >16 | GGG | Gly |
| RIT30A | >16 | GGG | Gly |
| RIT31 | >16 | GGG | Gly |
| RIT32 | >16 | GAA | Glu |
| RIT33 | >16 | GGG | Gly |
| RIT34 | >16 | GAG | Glu |
| RIT35 | >16 | GGG | Gly |
| RIT37 | >16 | GGG | Gly |
| RIT38 | >16 | AAG | Lys |
| RIT39 | >16 | GAA | Glu |
| RIT40 | >16 | GAG | Glu |
| RIT41 | >16 | AGG | Arg |
| RIT42 | >16 | GGG | Gly |
| RIT43 | >16 | GAA | Glu |
| RIT44 | >16 | GGG | Gly |
| RIT45 | >16 | GAA | Glu |
| RIT46 | >16 | GGG | Gly |
| RIT47 | >16 | GGG | Gly |
| RIT48 | >16 | GGG | Gly |
| RIT50A | >16 | GGG | Gly |
| RIT51 | >16 | AGG | Arg |
| RIT52 | >16 | GGG | Gly |
| RIT53 | >16 | GAA | Glu |
| RIT54 | >16 | GGG | Gly |
| RIT55 | >16 | AGG | Arg |
| RIT56 | >16 | GGG | Gly |
| Af41 | 0.25 | GGG | Gly |
| Af72 | >16 | GAG | Glu |
| Af90 | >16 | GGG | Gly |
| Br128 | >16 | GGG | Gly |
| Br130 | >16 | GAG | Glu |
| Br181 | 8.0 | GTG | Val |
| F/5211 | 0.5 | GGG | Gly |
| F/6919 | >16 | GGG | Gly |
| F/7075 | >16 | GAG | Glu |
| F/7763 | 1.0 | GGG | Gly |
| SO/3626 | 1.0 | GGG | Gly |
| SO/3827A | >16 | GTG | Val |
| SO/3827B | >16 | GTG | Val |
| SO/3829 | >16 | GTG | Val |
a
Data are from Mann et al. (26).
b
Additional mutation T164A resulting in an I55N amino acid change was revealed in the RIT19 strain by sequencing.