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. 2016 Dec 16;63(2):127–133. doi: 10.1262/jrd.2016-096

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©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 3. — MMP2 and MMP9 activity in the equine uterus during early- to mid-pregnancy. (A) Gelatin zymography analysis of MMP2 and MMP9 activity in the extracts from equine endometrial samples. In day 13 cyclic (C13) and day 13 pregnant (P13) uteri, samples from left (L3) or right (R3) endometrium were pooled and analyzed. In days 19 (P19) and 25 (P25) pregnant uteri, samples from non-gravid (N3) or gravid (G3) endometrium were separately analyzed. In days 60 to 88 and 96 to 131 pregnant uteri, three independent samples were mixed and analyzed. (B-a) Gelatin zymography analysis of MMP2 and MMP9 activity in the luminal fluid on day 25 of pregnancy. (B-b) Western blot analysis of LCN2 in the luminal fluid in non-reduced (–ME) and reduced (+ME) conditions on day 25 of pregnancy is also presented. A representative image is shown.