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. 2016 Dec 11;63(2):135–142. doi: 10.1262/jrd.2016-130

Fig. 2.

Fig. 2.

Validation of TEAD4 siRNA at the morula stage on Day 5. (A) Relative abundance (mean ± SEM) of TEAD4 transcripts in uninjected (n = 6), control siRNA-injected (n = 5), or TEAD4 siRNA-injected embryos (n = 6). a, b Different characters indicate a significant difference (P < 0.01). (B) Representative images of TEAD4 immunofluorescent staining of morula-stage uninjected, control siRNA-injected, or TEAD4 siRNA-injected embryos. Embryos were labeled with TEAD4 antibody (green) and DAPI for DNA (blue). Scale bars, 50 µm.