Table 1. Primers and siRNA sequences.
Name | Nucleotide sequences (5ʹ–3ʹ)* | Annealing temperature | Fragment size | GenBank |
(ºC) | (bp) | accession no. | ||
TEAD4 | F- AAGTTCTGGGCAGACCTCAA | 60 | 249 | XM_010827947.1 |
R- GTGCTTCAGCTTGTGGATGA | ||||
CDX2 | F- GCCACCATGTACGTGAGCTACC | 55 | 140 | NM_001206299.1 |
R- ACATGGTATCCGCCGTAGTCCGG | ||||
GATA3 | F- ATGAAACCGAAACCCGATGG | 60 | 185 | NM_001076804 |
R- TTCACAGCACTAGAGAGACC | ||||
Oct-4 | F- GGTTCTCTTTGGAAAGGTGTTC | 52 | 314 | AF022987.1 |
R- ACACTCGGACCACGTCTTTC | ||||
NANOG | F- AATTCCCAGCAGCAAATCAC | 55 | 215 | DQ069776 |
R- CCCTTCCCTCAAATTGACAC | ||||
IFNT | F- GCAGTGCTTCAACCTCTTCC | 62 | 155 | AF238611.1 |
R- TCCTTCCCATGTCAGAGTCC | ||||
Histone H2A | F- AGGACGACTAGCCATGGACGTGTG | 60 | 209 | NM_174809 |
R- CCACCACCAGCAATTGTAGCCTTG | ||||
TEAD4 siRNA | S- GCUACGAGAAUGGCCACUATT | N/A | N/A | N/A |
AS- UAGUGGCCAUUCUCGUAGCTT | N/A | N/A | N/A |
* F, forward; R, reverse; S, sense strand; AS, antisense strand.