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. 2016 Dec 11;63(2):135–142. doi: 10.1262/jrd.2016-130

Table 1. Primers and siRNA sequences.

Name Nucleotide sequences (5ʹ–3ʹ)* Annealing temperature Fragment size GenBank
(ºC) (bp) accession no.
TEAD4 F- AAGTTCTGGGCAGACCTCAA 60 249 XM_010827947.1
R- GTGCTTCAGCTTGTGGATGA
CDX2 F- GCCACCATGTACGTGAGCTACC 55 140 NM_001206299.1
R- ACATGGTATCCGCCGTAGTCCGG
GATA3 F- ATGAAACCGAAACCCGATGG 60 185 NM_001076804
R- TTCACAGCACTAGAGAGACC
Oct-4 F- GGTTCTCTTTGGAAAGGTGTTC 52 314 AF022987.1
R- ACACTCGGACCACGTCTTTC
NANOG F- AATTCCCAGCAGCAAATCAC 55 215 DQ069776
R- CCCTTCCCTCAAATTGACAC
IFNT F- GCAGTGCTTCAACCTCTTCC 62 155 AF238611.1
R- TCCTTCCCATGTCAGAGTCC
Histone H2A F- AGGACGACTAGCCATGGACGTGTG 60 209 NM_174809
R- CCACCACCAGCAATTGTAGCCTTG
TEAD4 siRNA S- GCUACGAGAAUGGCCACUATT N/A N/A N/A
AS- UAGUGGCCAUUCUCGUAGCTT N/A N/A N/A

* F, forward; R, reverse; S, sense strand; AS, antisense strand.