Table 3. Effect of TEAD4 siRNA injection on in vitro development under 21% O2 conditions*.
Treatment | Number of embryos cultured | No. (%)† of embryos developed to |
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Day 2 | Day 4 | Day 5 | Day 6 | Day 7 | Day 8 |
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2-cell ≤ | 16-cell ≤ | 32-cell ≤ | Morula ≤ | Blastocyst ≤ | Blastocyst ≤ | Hatching blastocyst ≤ | ||
Uninjected | 228 | 198 (86.8) | 149 (65.4) | 106 (46.5) | 99 (43.4) | 93 (40.8) | 84 (36.8) | 12 (5.3) |
Control siRNA | 212 | 179 (84.4) | 131 (61.8) | 106 (50.0) | 99 (46.7) | 102 (48.1) | 94 (44.3) | 5 (2.4) |
TEAD4 siRNA | 217 | 196 (90.3) | 143 (65.9) | 117 (53.9) | 104 (47.9) | 88 (40.6) | 93 (42.9) | 9 (4.1) |
* Experiments were replicated six times. † Percentages of the number of embryos cultured.