. 2017 Feb 5;63(2):175–183. doi: 10.1262/jrd.2016-142

Table 1. Effect of heparin concentration on cleavage after IVF using sorted and unsorted sperm.

Heparin treatment (µg/ml)	Cleavage (%) at 44–48 h post IVF (mean ± SEM)
	Bull A		Bull B		Bull C		Bull D
	Sorted	Unsorted	Sorted	Unsorted	Sorted	Unsorted	Sorted	Unsorted
0	10.2 ± 1.2 ^a	12.1 ± 2.1 ^a	23.2 ± 5.3 ^a	21.3 ± 4.5 ^a	10.9 ± 3.2 ^a	61.9 ± 6.6 ^a	9.1 ± 4.5 ^a	23.5 ± 2.3 ^a
2.5	18.8 ± 1.8 ^a	13.9 ± 6.9 ^a	24.9 ± 3.2 ^a	20.1 ± 2.4 ^a	16.5 ± 3.4 ^a	65.9 ± 8.9 ^a	15.6 ± 4.3 ^a	23.3 ± 3.4 ^a
5	24.2 ± 3.2 ^a	18.3 ± 5.7 ^a	28.6 ± 4.5 ^a	19.7 ± 0.7 ^a	19.4 ± 6.6 ^a	58.9 ± 5.7 ^a	19.1 ± 6.3 ^a	28.9 ± 3.9 ^a
10	40.7 ± 6.3 ^b	31.9 ± 3.6 ^b	36.3 ± 8.1 ^b	20.6 ± 5.5 ^a	48.4 ± 3.3 ^b	61.5 ± 1.5 ^a	18.3 ± 4.3 ^a	31.4 ± 4.5 ^a,b
20	45.3 ± 1.2 ^b	47.4 ± 3.4 ^c	33.9 ± 3.1 ^b	50.2 ± 2.3 ^b	47.7 ± 4.5 ^b	63.9 ± 3.5 ^a	28.1 ± 2.5 ^b	37.6 ± 3.2 ^b
40	52.5 ± 5.5 ^b	61.3 ± 3.4 ^d	47.4 ± 6.5 ^b	48.2 ± 6.7 ^b	54.1 ± 7.2 ^b	66.7 ± 1.5 ^a	25.6 ± 3.3 ^b	50.9 ± 4.8 ^c
60	52.8 ± 4.5 ^b	67.1 ± 5.5 ^d	34.6 ± 8.4 ^b	49.2 ± 6.6 ^b	47.2 ± 4.3 ^b	79.5 ± 7.5 ^a	28.9 ± 3.4 ^b	50.8 ± 4.5 ^c
80	57.4 ± 5.4 ^b	65.3 ± 4.5 ^d	42.7 ± 3.2 ^b	53.9 ± 4.5 ^b	42.1 ± 8.6 ^b	71.1 ± 6.7 ^a	25.1 ± 4.5 ^b	53.4 ± 3.9 ^c
100	49.2 ± 11^b	56.7 ± 3.2 ^d	39.2 ± 4.5 ^b	48.9 ± 7.2 ^b	38.5 ± 4.5 ^b	71.9 ± 5.3 ^a	30.6 ± 5.4 ^b	58.3 ± 4.5 ^c

Different letters (a, b, c, d) within the same column were significantly different, P < 0.05.