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Levels of cGMP in cumulus-oocyte complex (COC; A) and denuded oocyte (DO; B) derived from middle and small follicles (MF and SF, respectively) at 0, 10, 20, and 24 h after the start of maturation in vitro. Different letters indicate statistical significance (P < 0.05, 3–6 replicated trials). The numbers in the parentheses indicate the total number of COCs or DOs assayed.
