Table 2. Meiotic stages of MF- and SF-derived oocytes following in vitro maturation culture for 44 h.
Origin of COCs | No. of oocytes examined | No. (%) of oocytes at the stage of |
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GV | Pro M-I | M-I | A/T-I | M-II | ||
MF | 198 | 12 (6.1 ± 2.0) a | 6 (3.0 ± 1.2) | 23 (11.6 ± 2.6) a | 0 | 157 (79.3 ± 4.0) a |
SF | 195 | 32 (16.4 ± 2.3) b | 12 (6.2 ± 1.3) | 40 (20.6 ± 3.2) b | 5 (2.6 ± 1.4) | 106 (54.2 ± 3.3) b |
Data are shown as means ± SEM from five replicated trials. a,b Values with different superscripts within columns are significantly different (P < 0.05). MF, middle follicles; SF, small follicles; COCs, cumulus-oocyte complexes; GV, germinal vesicle; Pro M-I, pro-metaphase-I; M-I, metaphase-I; A/T-I, anaphase-I and telophase-I; M-II, metaphase-II.