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. 2017 Apr 24;8:74. doi: 10.3389/fendo.2017.00074

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Copyright © 2017 Sung Hsieh, Chung, Allen, Ranganathan, Habbouche, Cholok, Butts, Kaura, Tiruvannamalai-Annamalai, Breuler, Priest, Loder, Li, Li, Stegemann, Kunkel and Levi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunostaining of cytokines in the heterotopic ossification (HO) site. (A) Images show immunofluorescence staining of indicated cytokines (green) at the site of injury at 48 h and 1 week post-burn/tenotomy. (B) Images show immunofluorescence staining of indicated cytokines (green) HO anlagen of the genetic model NFAT-cre caACVR with corresponding DAPI overlays. Arrows indicate examples of positive staining. Scale bars indicate 50 µm.